Hypoxanthine guanine phosphoribosyltransferase 1, a target of miR-125b-5p, promotes cell proliferation and invasion in head and neck squamous cell carcinoma

The mechanism of hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) upregulation and its function in head neck squamous cell carcinoma (HNSCC) remains obscure. Herein, the expression HPRT1 underlying HNSCC were explored. Firstly, prognostic values simultaneously validated using bioinformatic analysis quantitative real-time PCR (qRT-PCR), immunohistochemistry staining with local samples. effects knockdown on proliferation invasion cells detected counting kit-8 (CCK-8), plate clone formation, Transwell invasion, nude mouse xenograft model assays. Moreover, miRNA targeting was dual-luciferase report assay, qRT-PCR Western blot analysis. functions dependence further investigated HNSCC. results indicated that highly expressed tissues cells, which positively correlated advanced tumor progression predicted poor prognosis patients markedly inhibited both vitro vivo. MiR-125b-5p, downregulated a favorable outcome for patients, directly targeted expression, subsequently suppressed Collectively, present study demonstrates upregulation, at least partially caused by miR-125b-5p downregulation, could promote malignant HNSCC, highlighting potential application miR-125b-5p/HPRT1 axis as novel indicator target diagnosis treatment